Article
A conserved Polϵ binding module in Ctf18-RFC is required for S-phase checkpoint activation downstream of Mec1.
Nucleic acids research - 15 Oct 2015
García-Rodríguez Luis J, De Piccoli Giacomo, Marchesi Vanessa, Jones Richard C, Edmondson Ricky D, Labib Karim
Abstract excerpt
Defects during chromosome replication in eukaryotes activate a signaling pathway called the S-phase checkpoint, which produces a multifaceted response that preserves genome integrity at stalled DNA replication forks. Work with budding yeast showed that the 'alternative clamp loader' known as Ctf18-RFC acts by an unknown mechanism to activate the checkpoint kinase Rad53, which then mediates much of the checkpoint...
Topics
- DNA Polymerase II
- DNA Replication
- DNA-Binding Proteins
- DNA-Directed DNA Polymerase
- Humans
- Intracellular Signaling Peptides and Proteins
- Multienzyme Complexes
- Mutation
- Protein Binding
- Protein Serine-Threonine Kinases
- Replication Protein C
- S Phase Cell Cycle Checkpoints