Article
[Quality evaluation and clinical applicability of pyrosequencing assay kit for detecting hepatitis B virus resistance].
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology - 1 Jun 2015
Chen Jian, Liu Ying, Zheng Zhidan, Shen Sheng, Sui Shaofei, Chen Hua, Zhou Bin, Sun Jian
Abstract excerpt
OBJECTIVE: To evaluate the quality and clinical applicability of pyrosequencing assay kit for detecting hepatitis B virus resistance (HBV DRT). METHODS: Serial dilutions of the International Standard for HBV DNA were used to test the detection limit of the PCR for HBV DRT. Plasmids containing the either a wild-type (WT) copy or one of 10 mutant (MT) copies of the HBV RT gene were used to prepare a series of...
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