Article
Evidence of the crucial role of the linker domain on the catalytic activity of human topoisomerase I by experimental and simulative characterization of the Lys681Ala mutant.
Nucleic acids research - 1 Nov 2009
Fiorani Paola, Tesauro Cinzia, Mancini Giordano, Chillemi Giovanni, D'Annessa Ilda, Graziani Grazia, Tentori Lucio, Muzi Alessia, Desideri Alessandro
Abstract excerpt
The functional and structural-dynamical properties of the Lys681Ala mutation in the human topoisomerase IB linker domain have been investigated by catalytic assays and molecular dynamics simulation. The mutant is characterized by a comparable cleavage and a strongly reduced religation rate when compared to the wild type protein. The mutant also displays perturbed linker dynamics, as shown by analysis of the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.