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Article

Locking the DNA topoisomerase I protein clamp inhibits DNA rotation and induces cell lethality.

Proceedings of the National Academy of Sciences of the United States of America - 25 Nov 2003

Woo Michael H, Losasso Carmen, Guo Hong, Pattarello Luca, Benedetti Piero, Bjornsti Mary-Ann

Abstract excerpt

Eukaryotic DNA topoisomerase I (Top1) is a monomeric protein clamp that functions in DNA replication, transcription, and recombination. Opposable "lip" domains form a salt bridge to complete Top1 protein clamping of duplex DNA. Changes in DNA topology are catalyzed by the formation of a transient phosphotyrosyl linkage between the active-site Tyr-723 and a single DNA strand. Substantial protein domain movements...

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Identifiers

PMID: 14585933
DOI: 10.1073/pnas.2235886100
PMCID: PMC283496

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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