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Article

Reconstitution of TCA cycle with DAOCS to engineer Escherichia coli into an efficient whole cell catalyst of penicillin G.

Proceedings of the National Academy of Sciences of the United States of America - 11 Aug 2015

Lin Baixue, Fan Keqiang, Zhao Jian, Ji Junjie, Wu Linjun, Yang Keqian, Tao Yong

Abstract excerpt

Many medically useful semisynthetic cephalosporins are derived from 7-aminodeacetoxycephalosporanic acid (7-ADCA), which has been traditionally made by the polluting chemical method. Here, a whole-cell biocatalytic process based on an engineered Escherichia coli strain expressing 2-oxoglutarate-dependent deacetoxycephalosporin C synthase (DAOCS) for converting penicillin G to G-7-ADCA is developed. The major...

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Identifiers

PMID: 26216972
DOI: 10.1073/pnas.1502866112
PMCID: PMC4538657

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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