Article
Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria.
Nature communications - 23 Jul 2021
Liu Jiaqi, Zhou Haibo, Yang Zhiyu, Wang Xue, Chen Hanna, Zhong Lin, Zheng Wentao, Niu Weijing, Wang Sen, Ren Xiangmei, Zhong Guannan, Wang Yan, Ding Xiaoming, Müller Rolf, Zhang Youming, Bian Xiaoying
Abstract excerpt
Heterologous expression of biosynthetic gene clusters (BGCs) avails yield improvements and mining of natural products, but it is limited by lacking of more efficient Gram-negative chassis. The proteobacterium Schlegelella brevitalea DSM 7029 exhibits potential for heterologous BGC expression, but its cells undergo early autolysis, hindering further applications. Herein, we rationally construct DC and DT series...
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