Article
[Evaluation of penicillin expandase mutants and complex substrate inhibition characteristics at high concentrations of penicillin G].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Dec 2015
Wu Linjun, Fan Keqiang, Ji Junjie, Yang Keqian
Abstract excerpt
Penicillin expandase, also known as deacetoxycephalosporin C synthase (DAOCS), is an essential enzyme involved in cephalosporin C biosynthesis. To evaluate the catalytic behaviors of penicillin expandase under high penicillin G concentration and to identify mutants suitable for industrial applications, the specific activities of wild-type DAOCS and several mutants with increased activities toward penicillin G...
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