Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Construction of recombinant Escherichia coli D11/pMSTO and its use in enzymatic preparation of 7-aminocephalosporanic acid in one pot.

Journal of biotechnology - 1 May 2007

Zheng Huabao, Zhu Tongbo, Chen Jun, Zhao Yuhua, Jiang Weihong, Zhao Guoping, Yang Sheng, Yang Yunliu

Abstract excerpt

The main drawback in the industrial production of 7-aminocephalosporanic acid is the accumulation of intermediate (AKA-7-ACA) and destruction of substrate (cephalosporin C) catalyzed by catalase and beta-lactamase. To overcome the adverse effect of these enzymes on the conversion process, Escherichia coli D11 with mutation of katG, katE and ampC genes was constructed by P1 phage transduction, which enabled it not...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to beta-Lactamases
Share to Biotechnology

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Catalase

Share to Cephalosporins

Share to Chromatography, High Pressure Liquid

Share to DNA, Recombinant

Share to Escherichia coli Proteins

Share to Transduction, Genetic

Source

Identifiers

PMID: 17349708
DOI: 10.1016/j.jbiotec.2007.01.021

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Construction of recombinant Escherichia coli D11/pMSTO and its use in enzymatic preparation of 7-aminocephalosporanic acid in one pot.PMID 17349708Read ↗

Reconstitution of TCA cycle with DAOCS to engineer Escherichia coli into an efficient whole cell catalyst of penicillin G.Semantic proximity Read ↗

Overexpression of synthesized cephalosporin C acylase containing mutations in the substrate transport tunnel.Semantic proximity Read ↗

Evolution of an acylase active on cephalosporin C.Semantic proximity Read ↗

Optimization of Cephalosporin C Acylase Expression in Escherichia coli by High-Throughput Screening a Constitutive Promoter Mutant library.Semantic proximity Read ↗

Engineering Escherichia coli for efficient production of 5-aminolevulinic acid from glucose.Semantic proximity Read ↗

A highly active adipyl-cephalosporin acylase obtained via rational randomization.Semantic proximity Read ↗

Relevant double mutations in bioengineered Streptomyces clavuligerus deacetoxycephalosporin C synthase result in higher binding specificities which improve penicillin bioconversion.Semantic proximity Read ↗

Efficient cascade synthesis of ampicillin from penicillin G potassium salt using wild and mutant penicillin G acylase from Alcaligenes faecalis.Semantic proximity Read ↗

Construction of a high-efficiency GjCCD4a mutant and its application for de novo biosynthesis of five crocins in Escherichia coli.Semantic proximity Read ↗

Breeding and characterization of amino acid-analogue-resistant mutants of Arthrospira platensis.Semantic proximity Read ↗

Production of d-Tagatose by Whole-Cell Conversion of Recombinant Bacillus subtilis in the Absence of AntibioticsSemantic proximity Read ↗

Strain improvement of Lentzea sp. 7887 for higher yield per unit volume on hydroxylation of cyclosporine derivative FR901459.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.