Article
Cooperative transitions of isolated Escherichia coli nucleoids: implications for the nucleoid as a cellular phase.
Journal of structural biology - 1 Feb 2006
Zimmerman Steven B
Abstract excerpt
The genomic DNA of Escherichia coli occurs in compact bodies known as nucleoids. Organization and structure of nucleoids are poorly understood. Compact, characteristically shaped, nucleoids isolated by the polylysine-spermidine procedure were visualized by DNA fluorescence microscopy. Treatment with urea or trypsin converted compact nucleoids to partially expanded forms. The transition in urea solutions was...
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