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Article

Aligning to the sample-specific reference sequence to optimize the accuracy of next-generation sequencing analysis for hepatitis B virus.

Hepatology international - 1 Jan 2016

Liu Wen-Chun, Lin Chih-Peng, Cheng Chun-Pei, Ho Cheng-Hsun, Lan Kuo-Lun, Cheng Ji-Hong, Yen Chia-Jui, Cheng Pin-Nan, Wu I-Chin, Li I-Chen, Chang Bill Chia-Han, Tseng Vincent S, Chiu Yen-Cheng, Chang Ting-Tsung

Abstract excerpt

BACKGROUND: Hepatitis B virus (HBV) quasispecies are crucial in the pathogenesis of chronic liver disease. Next-generation sequencing (NGS) is powerful for identifying viral quasispecies. To improve mapping quality and single nucleotide variant (SNV) calling accuracy in the NGS analysis of HBV, we compared different mapping references, including the sample-specific reference sequence, same genotype sequences and...

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Share to Base Sequence
Share to DNA, Viral

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Hepatitis B, Chronic

Share to Hepatitis B virus

Share to High-Throughput Nucleotide Sequencing

Share to Reference Values

Share to Sequence Alignment

Share to Sequence Analysis, DNA

Source

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Identifiers

PMID: 26208819
DOI: 10.1007/s12072-015-9645-x
PMCID: PMC4722079

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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