Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Integrating nested PCR with high-throughput sequencing to characterize mutations of HBV genome in low viral load samples.

Medicine - 1 Jul 2017

Wang Xianjun, Xu Lihui, Chen Yueming, Liu Anbing, Wang Liqian, Xu Peisong, Liu Yunhui, Li Lei, Meng Fei

Abstract excerpt

Due to the low viral load of hepatitis B virus (HBV) in plasma samples, conventional techniques have limitations to the detection of antiviral resistance mutations. To solve the problem, we developed a fast, highly sensitive, and accurate method to sequence the HBV whole-genome sequencing in plasma samples which had various viral loads from very low to high.Twenty-one plasma samples were collected from patients...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Child, Preschool
Share to DNA, Viral

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Drug Resistance, Viral

Share to Enzyme-Linked Immunosorbent Assay

Share to Hepatitis B, Chronic

Share to Hepatitis B virus

Share to Viral Load

Share to Young Adult

Source

Open in PubMed Open full text

Identifiers

PMID: 28746207
DOI: 10.1097/MD.0000000000007588
PMCID: PMC5627833

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Integrating nested PCR with high-throughput sequencing to characterize mutations of HBV genome in low viral load samples.PMID 28746207Read ↗

Next-generation sequencing for the diagnosis of hepatitis B: current status and future prospects.Semantic proximity Read ↗

Applications of next-generation sequencing analysis for the detection of hepatocellular carcinoma-associated hepatitis B virus mutations.Semantic proximity Read ↗

Insights From Deep Sequencing of the HBV Genome—Unique, Tiny, and MisunderstoodSemantic proximity Read ↗

A New Method for Next-Generation Sequencing of the Full Hepatitis B Virus Genome from A Clinical Specimen: Impact for Virus GenotypingSemantic proximity Read ↗

Analysis of hepatitis B virus genotyping and drug resistance gene mutations based on massively parallel sequencing.Semantic proximity Read ↗

Development and Validation of a Template-Independent Next-Generation Sequencing Assay for Detecting Low-Level Resistance-Associated Variants of Hepatitis C Virus.Semantic proximity Read ↗

Deep Sequencing of Hepatitis B Virus Reveals Clinically Relevant Low-Frequency Variants Among People Living with HIV in BotswanaSemantic proximity Read ↗

Applying next-generation sequencing to unravel the mutational landscape in viral quasispecies.Semantic proximity Read ↗

Different Variants in Reverse Transcriptase Domain Determined by Ultra-deep Sequencing in Treatment-naïve and Treated Indonesian Patients Infected with Hepatitis B Virus.Semantic proximity Read ↗

A Next-Generation Sequencing-Based Platform for Quantitative Detection of Hepatitis B Virus Pre-S Mutants in Plasma of Hepatocellular Carcinoma PatientsSemantic proximity Read ↗

HIV-1 Genotypic Resistance Testing Using Sanger and Next-Generation Sequencing in Adults with Low-Level Viremia in ChinaSemantic proximity Read ↗

Comparison of next-generation sequencing and clone-based sequencing in analysis of hepatitis B virus reverse transcriptase quasispecies heterogeneity.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.