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Article

Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells.

Nature methods - 1 Aug 2015

König Iwo, Zarrine-Afsar Arash, Aznauryan Mikayel, Soranno Andrea, Wunderlich Bengt, Dingfelder Fabian, Stüber Jakob C, Plückthun Andreas, Nettels Daniel, Schuler Benjamin

Abstract excerpt

Single-molecule methods have become widely used for quantifying the conformational heterogeneity and structural dynamics of biomolecules in vitro. Their application in vivo, however, has remained challenging owing to shortcomings in the design and reproducible delivery of labeled molecules, the range of applicable analysis methods, and suboptimal cell culture conditions. By addressing these limitations in an...

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PMID: 26147918
DOI: 10.1038/nmeth.3475

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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