Article
Hyper sensitive protein detection by Tandem-HTRF reveals Cyclin D1 dynamics in adult mouse.
Scientific reports - 27 Oct 2015
Zampieri Alexandre, Champagne Julien, Auzemery Baptiste, Fuentes Ivanna, Maurel Benjamin, Bienvenu Frédéric
Abstract excerpt
We present here a novel method for the semi-quantitative detection of low abundance proteins in solution that is both fast and simple. It is based on Homogenous Time Resolved Förster Resonance Energy Transfer (HTRF), between a lanthanide labeled donor antibody and a d2 or XL665 labeled acceptor antibody that are both raised against different epitopes of the same target. This novel approach we termed...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
