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Direct high‐throughput amplification and sequencing of immunoglobulin genes from single human B cells

3 Jul 2015

Abstract excerpt

Single cell Ig gene amplification and sequencing has been widely used for the molecular and functional assessment of human antibody repertoires and has led to the identification of recombinant monoclonal antibodies with therapeutic potential against diverse pathogens 1-3. Due to the high reagent and Sanger sequencing costs, these antibody-cloning strategies are limited to the analysis of relatively small B-cell...

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PMID: 26138551
DOI: 10.1002/eji.201545526

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