Article
High-quality full-length immunoglobulin profiling with unique molecular barcoding.
Nature protocols - 1 Sept 2016
Turchaninova M A, Davydov A, Britanova O V, Shugay M, Bikos V, Egorov E S, Kirgizova V I, Merzlyak E M, Staroverov D B, Bolotin D A, Mamedov I Z, Izraelson M, Logacheva M D, Kladova O, Plevova K, Pospisilova S, Chudakov D M
Abstract excerpt
High-throughput sequencing analysis of hypermutating immunoglobulin (IG) repertoires remains a challenging task. Here we present a robust protocol for the full-length profiling of human and mouse IG repertoires. This protocol uses unique molecular identifiers (UMIs) introduced in the course of cDNA synthesis to control bottlenecks and to eliminate PCR and sequencing errors. Using asymmetric 400+100-nt paired-end...
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