Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Expression cloning of human B cell immunoglobulins.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013

Wardemann Hedda, Kofer Juliane

Abstract excerpt

The majority of lymphomas originate from B cells at the germinal center stage or beyond. Preferential selection of B cell clones by a limited set of antigens has been suggested to drive lymphoma development. However, little is known about the specificity of the antibodies expressed by lymphoma cells, and the role of antibody-specificity in lymphomagenesis remains elusive. Here, we describe a strategy to...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Antibodies, Monoclonal
Share to B-Lymphocytes
Share to Cloning, Molecular

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA, Complementary

Share to Flow Cytometry

Share to Genetic Vectors

Share to Immunoglobulins

Share to Reverse Transcriptase Polymerase Chain Reaction

Source

Identifiers

PMID: 23296959
DOI: 10.1007/978-1-62703-269-8_5

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Expression cloning of human B cell immunoglobulins.PMID 23296959Read ↗

High-throughput isolation of immunoglobulin genes from single human B cells and expression as monoclonal antibodiesSemantic proximity Read ↗

Direct high‐throughput amplification and sequencing of immunoglobulin genes from single human B cellsSemantic proximity Read ↗

Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloningSemantic proximity Read ↗

Rapid high-throughput cloning and stable expression of antibodies in HEK293 cellsSemantic proximity Read ↗

A tool kit for rapid cloning and expression of recombinant antibodiesSemantic proximity Read ↗

A general method for the selection of high-level scFv and IgG antibody expression by stably transfected mammalian cellsSemantic proximity Read ↗

Antibody Production Techniques and Antibody Types: Evolution, Methodologies, and ApplicationsSemantic proximity Read ↗

Brain Antibody Sequence Evaluation (BASE): an easy-to-use software for complete data analysis in single cell immunoglobulin cloningSemantic proximity Read ↗

Retrocyte Display® technology: generation and screening of a high diversity cellular antibody library.Semantic proximity Read ↗

Baculovirus expression cassette vectors for rapid production of complete human IgG from phage display selected antibody fragmentsSemantic proximity Read ↗

Target-selective homologous recombination cloning for high-throughput generation of monoclonal antibodies from single plasma cellsSemantic proximity Read ↗

Mammalian cell display for rapid screening scFv antibody therapySemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.