Unbiased quantification of immunoglobulin diversity at the DNA level with VDJ-seq.

Abstract excerpt

For high-throughput sequencing and quantification of immunoglobulin repertoires, most methodologies use RNA. However, output varies enormously between recombined genes due to different promoter strengths and differential activation of lymphocyte subsets, precluding quantitation of recombinants on a per-cell basis. To date, DNA-based approaches have used V gene primer cocktails, with substantial inherent biases....

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Genes, Immunoglobulin
• Share to Genetic Variation