Article
Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours.
Nature communications - 17 Jun 2015
Ince Tan A, Sousa Aurea D, Jones Michelle A, Harrell J Chuck, Agoston Elin S, Krohn Marit, Selfors Laura M, Liu Wenbin, Chen Ken, Yong Mao, Buchwald Peter, Wang Bin, Hale Katherine S, Cohick Evan, Sergent Petra, Witt Abigail, Kozhekbaeva Zhanna, Gao Sizhen, Agoston Agoston T, Merritt Melissa A, Foster Rosemary, Rueda Bo R, Crum Christopher P, Brugge Joan S, Mills Gordon B
Abstract excerpt
Currently available human tumour cell line panels consist of a small number of lines in each lineage that generally fail to retain the phenotype of the original patient tumour. Here we develop a cell culture medium that enables us to routinely establish cell lines from diverse subtypes of human ovarian cancers with >95% efficiency. Importantly, the 25 new ovarian tumour cell lines described here retain the...
Topics
- Carcinoma
- Cell Line, Tumor
- Cisplatin
- Culture Media
- Drug Screening Assays, Antitumor
- Female
- Gene Expression Profiling
- Heterografts
- Humans