Article
Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette.
Genetics - 1 Aug 2015
Dickinson Daniel J, Pani Ariel M, Heppert Jennifer K, Higgins Christopher D, Goldstein Bob
Abstract excerpt
A central goal in the development of genome engineering technology is to reduce the time and labor required to produce custom genome modifications. Here we describe a new selection strategy for producing fluorescent protein (FP) knock-ins using CRISPR/Cas9-triggered homologous recombination. We h...
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