Article
A novel suicide plasmid for efficient gene mutation in Listeria monocytogenes.
Plasmid - 1 Sept 2015
Abdelhamed Hossam, Lawrence Mark L, Karsi Attila
Abstract excerpt
Although several plasmids have been used in Listeria monocytogenes for generating mutants by allelic exchange, construction of L. monocytogenes mutants has been inefficient due to lack of effective selection markers for first and second recombination events. To address this problem, we have developed a new suicide plasmid, pHoss1, by using the pMAD plasmid backbone and anhydrotetracycline selection marker (secY...
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