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Article

Using a split luciferase assay (SLA) to measure the kinetics of cell-cell fusion mediated by herpes simplex virus glycoproteins.

Methods (San Diego, Calif.) - 15 Nov 2015

Saw Wan Ting, Matsuda Zene, Eisenberg Roselyn J, Cohen Gary H, Atanasiu Doina

Abstract excerpt

Herpes simplex virus (HSV) entry and cell-cell fusion require the envelope proteins gD, gH/gL and gB. We propose that receptor-activated conformational changes to gD activate gH/gL, which then triggers gB (the fusogen) into an active form. To study this dynamic process, we have adapted a dual split protein assay originally developed to study the kinetics of human immunodeficiency virus (HIV) mediated fusion. This...

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Identifiers

PMID: 26022509
DOI: 10.1016/j.ymeth.2015.05.021
PMCID: PMC4655157

Genes

SLA

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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