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Article

Identifying microbial fitness determinants by insertion sequencing using genome-wide transposon mutant libraries.

Nature protocols - 17 Nov 2011

Goodman Andrew L, Wu Meng, Gordon Jeffrey I

Abstract excerpt

Insertion sequencing (INSeq) is a method for determining the insertion site and relative abundance of large numbers of transposon mutants in a mixed population of isogenic mutants of a sequenced microbial species. INSeq is based on a modified mariner transposon containing MmeI sites at its ends, allowing cleavage at chromosomal sites 16-17 bp from the inserted transposon. Genomic regions adjacent to the...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 22094732
DOI: 10.1038/nprot.2011.417
PMCID: PMC3310428

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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