Article
Characterization of genome-wide ordered sequence-tagged Mycobacterium mutant libraries by Cartesian Pooling-Coordinate Sequencing.
Nature communications - 11 May 2015
Vandewalle Kristof, Festjens Nele, Plets Evelyn, Vuylsteke Marnik, Saeys Yvan, Callewaert Nico
Abstract excerpt
Reverse genetics research approaches require the availability of methods to rapidly generate specific mutants. Alternatively, where these methods are lacking, the construction of pre-characterized libraries of mutants can be extremely valuable. However, this can be complex, expensive and time consuming. Here, we describe a robust, easy to implement parallel sequencing-based method (Cartesian Pooling-Coordinate...
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