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Article

Synthesis of 2'-Fluoro RNA by Syn5 RNA polymerase.

Nucleic acids research - 18 Aug 2015

Zhu Bin, Hernandez Alfredo, Tan Min, Wollenhaupt Jan, Tabor Stanley, Richardson Charles C

Abstract excerpt

The substitution of 2'-fluoro for 2'-hydroxyl moieties in RNA substantially improves the stability of RNA. RNA stability is a major issue in RNA research and applications involving RNA. We report that the RNA polymerase from the marine cyanophage Syn5 has an intrinsic low discrimination against the incorporation of 2'-fluoro dNMPs during transcription elongation. The presence of both magnesium and manganese ions...

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PMID: 25897116
DOI: 10.1093/nar/gkv367
PMCID: PMC4538805

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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