Article
Multiplexed metagenome mining using short DNA sequence tags facilitates targeted discovery of epoxyketone proteasome inhibitors.
Proceedings of the National Academy of Sciences of the United States of America - 7 Apr 2015
Owen Jeremy G, Charlop-Powers Zachary, Smith Alexandra G, Ternei Melinda A, Calle Paula Y, Reddy Boojala Vijay B, Montiel Daniel, Brady Sean F
Abstract excerpt
In molecular evolutionary analyses, short DNA sequences are used to infer phylogenetic relationships among species. Here we apply this principle to the study of bacterial biosynthesis, enabling the targeted isolation of previously unidentified natural products directly from complex metagenomes. Our approach uses short natural product sequence tags derived from conserved biosynthetic motifs to profile biosynthetic...
Topics
- Computational Biology
- DNA
- Drug Design
- Drug Discovery
- Genetic Variation
- Genome
- Genome, Bacterial
- Geography
- Ketones
- Magnetic Resonance Spectroscopy