Article
Functional analysis of environmental DNA-derived type II polyketide synthases reveals structurally diverse secondary metabolites.
Proceedings of the National Academy of Sciences of the United States of America - 2 Aug 2011
Feng Zhiyang, Kallifidas Dimitris, Brady Sean F
Abstract excerpt
A single gram of soil is predicted to contain thousands of unique bacterial species. The majority of these species remain recalcitrant to standard culture methods, prohibiting their use as sources of unique bioactive small molecules. The cloning and analysis of DNA extracted directly from environmental samples (environmental DNA, eDNA) provides a means of exploring the biosynthetic capacity of natural bacterial...
Topics
- Aminoglycosides
- Anti-Bacterial Agents
- Bacteria
- DNA, Bacterial
- Gene Library
- Genetic Variation
- Methicillin-Resistant Staphylococcus aureus
- Molecular Sequence Data
- Molecular Structure
- Phylogeny
- Polyketide Synthases
- Sequence Analysis, DNA
- Soil Microbiology