Systematic High-Content Screening of Fluorescently Tagged Yeast Double Mutant Strains.

doi:10.1007/978-1-0716-1740-3_3

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Article

Systematic High-Content Screening of Fluorescently Tagged Yeast Double Mutant Strains.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Garadi Suresh Harsha, Mattiazzi Usaj Mojca

Abstract excerpt

We describe a protocol for high-content screening in budding yeast that can be used to study genetic interactions from a cell biological perspective. This approach can be used to map genetic interactions by monitoring one or more subcellular fluorescent markers of interest. In this case, changes in the morphology or abundance of a subcellular compartment, pathway or bioprocess are monitored in the background of a...

Topics

Mutation
Proteome
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins

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Open in PubMed

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PMID: 34590270
DOI: 10.1007/978-1-0716-1740-3_3

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