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A fluorescent tool set for yeast Atg proteins

22 May 2015

Abstract excerpt

Fluorescence microscopy of live cells is instrumental in deciphering the molecular details of autophagy. To facilitate the routine examination of yeast Atg proteins under diverse conditions, here we provide a comprehensive tool set, including (1) plasmids for the expression of GFP chimeras at endogenous levels for most Atg proteins, (2) RFP-Atg8 constructs with improved properties as a PAS marker, and (3)...

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PMID: 25998947
DOI: 10.1080/15548627.2015.1040971

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