Unwinding forward and sliding back: an intermittent unwinding mode of the BLM helicase.

Abstract excerpt

There are lines of evidence that the Bloom syndrome helicase, BLM, catalyzes regression of stalled replication forks and disrupts displacement loops (D-loops) formed during homologous recombination (HR). Here we constructed a forked DNA with a 3' single-stranded gap and a 5' double-stranded handle to partly mimic a stalled DNA fork and used magnetic tweezers to study BLM-catalyzed unwinding of the forked DNA. We...

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• BLM gene
• Mutation
• Protein Conformation
• RecQ Helicases