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In vitro reconstitution of indolmycin biosynthesis reveals the molecular basis of oxazolinone assembly

17 Feb 2015

Abstract excerpt

The bacterial tryptophanyl-tRNA synthetase inhibitor indolmycin features a unique oxazolinone heterocycle whose biogenetic origins have remained obscure for over 50 years. Here we identify and characterize the indolmycin biosynthetic pathway, using systematic in vivo gene inactivation, in vitro biochemical assays, and total enzymatic synthesis. Our work reveals that a phenylacetate-CoA ligase-like enzyme Ind3...

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PMID: 25730866
DOI: 10.1073/pnas.1419964112

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