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Article

Simultaneous use of in silico design and a correlated mutation network as a tool to efficiently guide enzyme engineering.

Chembiochem : a European journal of chemical biology - 23 Mar 2015

Nobili Alberto, Tao Yifeng, Pavlidis Ioannis V, van den Bergh Tom, Joosten Henk-Jan, Tan Tianwei, Bornscheuer Uwe T

Abstract excerpt

In order to improve the efficiency of directed evolution experiments, in silico multiple-substrate clustering was combined with an analysis of the variability of natural enzymes within a protein superfamily. This was applied to a Pseudomonas fluorescens esterase (PFE I) targeting the enantioselective hydrolysis of 3-phenylbutyric acid esters. Data reported in the literature for nine substrates were used for the...

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PMID: 25711719
DOI: 10.1002/cbic.201402665

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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