Enhancing prediction accuracy for enzyme activity engineering through sequence co-evolution and epistatic relationship modeling.

Abstract excerpt

Conventional enzyme engineering strategies, such as directed evolution with structure-based analysis, are limited by laborious workflows and by the need to screen extensive enzyme libraries. Computational models based on multiple sequence alignment, such as SCANEER, have streamlined these engineering strategies, enabling the successful prediction of single-site mutations that enhance enzyme activity. However,...

Topics

• Epistasis, Genetic
• Mutation
• Protein Engineering
• Directed Molecular Evolution
• Enzymes
• Evolution, Molecular