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Article

Directed evolution to re-adapt a co-evolved network within an enzyme.

Journal of biotechnology - 1 Jan 2012

Strafford John, Payongsri Panwajee, Hibbert Edward G, Morris Phattaraporn, Batth Sukhjeet S, Steadman David, Smith Mark E B, Ward John M, Hailes Helen C, Dalby Paul A

Abstract excerpt

We have previously used targeted active-site saturation mutagenesis to identify a number of transketolase single mutants that improved activity towards either glycolaldehyde (GA), or the non-natural substrate propionaldehyde (PA). Here, all attempts to recombine the singles into double mutants led to unexpected losses of specific activity towards both substrates. A typical trade-off occurred between soluble...

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Identifiers

PMID: 22154561
DOI: 10.1016/j.jbiotec.2011.11.017
PMCID: PMC3657141

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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