Article
High diversity in the TCR repertoire of GAD65 autoantigen-specific human CD4+ T cells.
Journal of immunology (Baltimore, Md. : 1950) - 15 Mar 2015
Eugster Anne, Lindner Annett, Catani Mara, Heninger Anne-Kristin, Dahl Andreas, Klemroth Sylvia, Kühn Denise, Dietz Sevina, Bickle Marc, Ziegler Anette-Gabrielle, Bonifacio Ezio
Abstract excerpt
Autoreactive CD4(+) T cells are an essential feature of type 1 diabetes mellitus. We applied single-cell TCR α- and β-chain sequencing to peripheral blood GAD65-specific CD4(+) T cells, and TCR α-chain next-generation sequencing to bulk memory CD4(+) T cells to provide insight into TCR diversity in autoimmune diabetes mellitus. TCRs obtained for 1650 GAD65-specific CD4(+) T cells isolated from GAD65 proliferation...
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