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Article

Optimizing phage-based mutant recovery and minimizing heat effect in the construction of transposon libraries in Staphylococcus aureus.

Scientific reports - 1 Oct 2024

Yousief Sally W, Abdelmalek Nader, Paglietti Bianca

Abstract excerpt

Staphylococcus aureus (S. aureus), particularly Methicillin-resistant S. aureus (MRSA), poses a significant global public health threat, necessitating advanced methodologies to enhance our understanding of this organism at the omics levels. This study introduces a refined protocol for constructing and curing high-density transposon mutant (tn-mutant) libraries in S. aureus, addressing the challenges associated...

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Share to Bacteriophages
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 39354068
DOI: 10.1038/s41598-024-73731-y
PMCID: PMC11445466

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