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Article

ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA.

Proceedings of the National Academy of Sciences of the United States of America - 25 Nov 2003

Perriman Rhonda, Barta Imre, Voeltz Gia K, Abelson John, Ares Manuel

Abstract excerpt

Stable addition of U2 small nuclear ribonucleoprotein (snRNP) to form the prespliceosome is the first ATP-dependent step in splicing, and it requires the DEXD/H box ATPase Prp5p. However, prespliceosome formation occurs without ATP in extracts lacking the U2 snRNP protein Cus2p. Here we show that...

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Share to Adenosine Triphosphate
Share to DEAD-box RNA Helicases
Share to Ribonucleoproteins, Small Nuclear

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 14610285
DOI: 10.1073/pnas.2036312100
PMCID: PMC283511

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026. Prepared record publication-14610285-2026-09-01.1. Manifest publication-graph-cohort-2026-09-01.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 1 Sept 2026.

Related research

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Research trail→

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ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA.PMID 14610285Read ↗

A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence.Semantic proximity Read ↗

Cus2 enforces the first ATP-dependent step of splicing by binding to yeast SF3b1 through a UHM-ULM interactionSemantic proximity Read ↗

Prp43p is a DEAH-box spliceosome disassembly factor essential for ribosome biogenesis.Semantic proximity Read ↗

Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA.Semantic proximity Read ↗

The DExD/H-box ATPase Prp2p destabilizes and proofreads the catalytic RNA core of the spliceosome.Semantic proximity Read ↗

Motifs IV and V in the DEAH box splicing factor Prp22 are important for RNA unwinding, and helicase-defective Prp22 mutants are suppressed by Prp8.Semantic proximity Read ↗

A splicing-dependent transcriptional checkpoint associated with prespliceosome formation.Semantic proximity Read ↗

A targeted bypass screen identifies Ynl187p, Prp42p, Snu71p, and Cbp80p for stable U1 snRNP/Pre-mRNA interaction.Semantic proximity Read ↗

Invariant U2 snRNA nucleotides form a stem loop to recognize the intron early in splicing.Semantic proximity Read ↗

Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.Semantic proximity Read ↗

Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing.Semantic proximity Read ↗

Structure-function analysis of the 5' end of yeast U1 snRNA highlights genetic interactions with the Msl5*Mud2 branchpoint-binding complex and other spliceosome assembly factors.Semantic proximity Read ↗

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