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Article

Mutation of purD and purF genes further attenuates Brucella abortus strain RB51.

Microbial pathogenesis - 1 Feb 2015

Truong Quang Lam, Cho Youngjae, Barate Abhijit Kashinath, Kim Suk, Watarai Masahisa, Hahn Tae-Wook

Abstract excerpt

In the present study, transposon mutagenesis was used to further attenuate Brucella abortus RB51 vaccine strain. Two purD and purF mutants were constructed, characterized and evaluated for attenuation via intracellular survival in murine macrophage-like RAW264.7 and HeLa cells, and by clearance in BALB/c mice. The purD and purF mutants showed significantly decreased intracellular survival, and complementation of...

Topics

Animals
Biosynthetic Pathways
Brucella abortus
Brucellosis
Cell Line
DNA Transposable Elements
Disease Models, Animal
Epithelial Cells
Genetic Complementation Test
Macrophages
Mice, Inbred BALB C

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis, Insertional

Mutation

Purines

Virulence

Virulence Factors

Source

Identifiers

PMID: 25546140
DOI: 10.1016/j.micpath.2014.12.003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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