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Article

Deletion of purE attenuates Brucella melitensis 16M for growth in human monocyte-derived macrophages.

Infection and immunity - 1 Sept 1995

Drazek E S, Houng H S, Crawford R M, Hadfield T L, Hoover D L, Warren R L

Abstract excerpt

We constructed a defined purine-auxotrophic mutant of Brucella melitensis 16M by chromosomal gene replacement. We electroporated B. melitensis 16M with suicide plasmids containing a kanamycin resistance cassette that replaced 226 bp at the carboxyl end of purE, the intergenic region, and 18 bases of the purK open reading frame. Recombinant B. melitensis delta purE201 required exogenous purines for growth on...

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Identifiers

PMID: 7642258
DOI: 10.1128/iai.63.9.3297-3301.1995
PMCID: PMC173454

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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