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Article

Deletion of purE attenuates Brucella melitensis 16M for growth in human monocyte-derived macrophages.

Infection and immunity - 1 Sept 1995

Drazek E S, Houng H S, Crawford R M, Hadfield T L, Hoover D L, Warren R L

Abstract excerpt

We constructed a defined purine-auxotrophic mutant of Brucella melitensis 16M by chromosomal gene replacement. We electroporated B. melitensis 16M with suicide plasmids containing a kanamycin resistance cassette that replaced 226 bp at the carboxyl end of purE, the intergenic region, and 18 bases...

Topics

Brucella melitensis
Carboxy-Lyases
Humans
Macrophages
Monocytes
Mutation
Purines

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 7642258
DOI: 10.1128/iai.63.9.3297-3301.1995
PMCID: PMC173454

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7642258-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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