Article
Immunohistochemical detection of the BRAF V600E mutant protein in colorectal neoplasms.
Applied immunohistochemistry & molecular morphology : AIMM - 1 Jul 2015
Vakiani Efsevia, Yaeger Rona, Brooke Sylvester, Zhou Yi, Klimstra David S, Shia Jinru
Abstract excerpt
Reliable assessment of the BRAF mutation status is becoming increasingly important in the clinical management of colorectal carcinomas (CRC). The aim of this study was to investigate the use of a recently developed mutation-specific antibody (VE1; SpringBio, Pleasanton, CA) to detect the BRAF V600E protein in paraffin tissue. We analyzed by immunohistochemistry (IHC) 117 cases that had been evaluated for BRAF...
Topics
- Amino Acid Substitution
- Antibodies, Neoplasm
- Antibody Affinity
- Antibody Specificity
- Colonic Polyps
- Colorectal Neoplasms
- Diagnosis, Differential
- Gene Expression
- Humans
- Immunohistochemistry