Article
Detection of the BRAF V600E mutation in colon carcinoma: critical evaluation of the imunohistochemical approach.
The American journal of surgical pathology - 1 Sept 2014
Lasota Jerzy, Kowalik Artur, Wasag Bartosz, Wang Zeng-Feng, Felisiak-Golabek Anna, Coates Tiffany, Kopczynski Janusz, Gozdz Stanislaw, Miettinen Markku
Abstract excerpt
Recently BRAF V600E mutant-specific antibody (clone VE1) became available to immunohistochemically pinpoint the occurrence of these BRAF-mutant proteins in different tumors, such as colon carcinoma. Detection of BRAF mutations is important for the accurate application of targeted therapy against BRAF serine-threonine kinase activation. In this study, we evaluated 113 colon carcinomas including 95 primary and 27...
Topics
- Adenocarcinoma
- Adult
- Aged
- Automation, Laboratory
- Biomarkers, Tumor
- Biopsy
- Colonic Neoplasms
- DNA Mismatch Repair
- DNA Mutational Analysis
- Female