Article
Ultra-deep sequencing confirms immunohistochemistry as a highly sensitive and specific method for detecting BRAF V600E mutations in colorectal carcinoma.
Virchows Archiv : an international journal of pathology - 1 Nov 2013
Rössle Matthias, Sigg Michèle, Rüschoff Jan H, Wild Peter J, Moch Holger, Weber Achim, Rechsteiner Markus P
Abstract excerpt
The activating BRAF (V600) mutation is a well-established negative prognostic biomarker in metastatic colorectal carcinoma (CRC). A recently developed monoclonal mouse antibody (clone VE1) has been shown to detect reliably BRAF (V600E) mutated protein by immunohistochemistry (IHC). In this study,...
Topics
- Adult
- Aged
- Aged, 80 and over
- Animals
- Antibodies, Monoclonal
- Biomarkers, Tumor
- Colorectal Neoplasms
- Female
- High-Throughput Nucleotide Sequencing
- Humans
- Immunohistochemistry
- Male
- Mice
- Middle Aged
- Mutation
- Proto-Oncogene Proteins B-raf