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Generating Targeted Mutants Using a Riboswitch to Control Plasmid Behavior During Allelic Exchange Mutagenesis.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2026

Brehm Joshua N, Sorg Joseph A

Abstract excerpt

Efficient and markerless allele-coupled exchange (ACE) mutagenesis is possible in a wild-type C. difficile background by using a synthetic riboswitch to indirectly control replication of the mutagenesis plasmid. Controlling plasmid replication gives the user the ability to introduce selective pressure for recombination of the mutagenesis plasmid into the chromosome (integration) followed by recombination of...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 42096146
DOI: 10.1007/978-1-0716-5328-9_2

Genes

ACE

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

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