Article
Chromosomal engineering of Clostridium perfringens using group II introns.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2008
Gupta Phalguni, Chen Yue
Abstract excerpt
Clostridium perfringens is a major natural pathogen of human and domestic animals owing to the production of multiple toxins. Defined clostridial mutants are essential for studying the role of toxins in disease pathogenesis. However, it has been very difficult to introduce mutations into C. perfringens. We recently developed a clostridia-modified targetron that can specifically and efficiently inactivate C....
Topics
- Animals
- Bacterial Toxins
- Base Sequence
- Blotting, Southern
- Calcium-Binding Proteins
- Chromosomes, Bacterial
- Cloning, Molecular
- Clostridium perfringens
- DNA Primers
- Escherichia coli
- Gene Targeting
- Genes, Bacterial
- Genetic Engineering
- Humans
- Introns
- Mutagenesis, Insertional
- Mutation
- Plasmids