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Article

Construction and analysis of chromosomal Clostridium difficile mutants.

Molecular microbiology - 1 Sept 2006

O'Connor Jennifer R, Lyras Dena, Farrow Kylie A, Adams Vicki, Powell David R, Hinds Jason, Cheung Jackie K, Rood Julian I

Abstract excerpt

Clostridium difficile is an emerging nosocomial pathogen of increasing importance and virulence but our ability to study the molecular mechanisms underlying the pathogenesis of C. difficile-associated disease has been limited because of a lack of tools for its genetic manipulation. We have now developed a reproducible method for the targeted insertional inactivation of chromosomal C. difficile genes. The approach...

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Share to Chromosomes, Bacterial
Share to Clostridioides difficile

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Electrophoretic Mobility Shift Assay

Share to Gene Expression Regulation, Bacterial

Share to Genetic Complementation Test

Share to Mutagenesis, Insertional

Share to Oligonucleotide Array Sequence Analysis

Share to Sequence Homology, Amino Acid

Source

Identifiers

PMID: 16925561
DOI: 10.1111/j.1365-2958.2006.05315.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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