Article
Generation of a single chain antibody variable fragment (scFv) to sense selectively RhoB activation.
PloS one - 1 Jan 2014
Chinestra Patrick, Olichon Aurélien, Medale-Giamarchi Claire, Lajoie-Mazenc Isabelle, Gence Rémi, Inard Cyril, Ligat Laetitia, Faye Jean-Charles, Favre Gilles
Abstract excerpt
Determining the cellular level of activated form of RhoGTPases is of key importance to understand their regulatory functions in cell physiopathology. We previously reported scFvC1, that selectively bind to the GTP-bound form of RhoA, RhoB and RhoC. In this present study we generate, by molecular evolution, a new phage library to isolate scFvs displaying high affinity and selectivity to RhoA and RhoB. Using phage...
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