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A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm

22 Nov 2007

Abstract excerpt

BACKGROUND: Intrabodies are defined as antibody molecules which are ectopically expressed inside the cell. Such intrabodies can be used to visualize or inhibit the targeted antigen in living cells. However, most antibody fragments cannot be used as intrabodies because they do not fold under the reducing conditions of the cell cytosol and nucleus. RESULTS: We describe the construction and validation of a large...

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PMID: 18034894
DOI: 10.1186/1472-6750-7-81

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