Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Isolation of trans-acting genes that enhance soluble expression of scFv antibodies in the E. coli cytoplasm by lambda phage display.

Journal of immunological methods - 10 Apr 2007

Levy Raphael, Molineux Ian J, Iverson Brent L, Georgiou George

Abstract excerpt

Functional antibody fragments with native disulfide bonds can be expressed in Escherichia coli trxB gor mutant strains having an oxidizing cytoplasm that allows the formation of disulfide bonds. However, expression yields in the cytoplasm are generally lower than those obtained by secretion into the periplasm. We developed a novel methodology for the screening of genomic DNA fragments that enhance expression...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacteriophage lambda
Share to Capsid Proteins

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Cytoplasm

Share to Genomic Library

Share to Immunoglobulin Fab Fragments

Share to Peptide Library

Share to Recombinant Fusion Proteins

Share to Thioredoxins

Source

Identifiers

PMID: 17328908
DOI: 10.1016/j.jim.2007.01.017

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Isolation of trans-acting genes that enhance soluble expression of scFv antibodies in the E. coli cytoplasm by lambda phage display.PMID 17328908Read ↗

A scFv antibody mutant isolated in a genetic screen for improved export via the twin arginine transporter pathway exhibits faster folding.Semantic proximity Read ↗

Production of correctly folded Fab antibody fragment in the cytoplasm of Escherichia coli trxB gor mutants via the coexpression of molecular chaperones.Semantic proximity Read ↗

A microbial expression system for high-level production of scFv HIV-neutralizing antibody fragments in Escherichia coliSemantic proximity Read ↗

High level production of functional antibody Fab fragments in an oxidizing bacterial cytoplasm.Semantic proximity Read ↗

Functional expression and display of an antibody Fab fragment in Escherichia coli: study of vector designs and culture conditionsSemantic proximity Read ↗

Efficient isolation of soluble intracellular single-chain antibodies using the twin-arginine translocation machinery.Semantic proximity Read ↗

In vivo drafting of single-chain antibodies for regulatory duty on the sigma54-promoter Pu of the TOL plasmid.Semantic proximity Read ↗

An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display librariesSemantic proximity Read ↗

Efficient expression of full-length antibodies in the cytoplasm of engineered bacteriaSemantic proximity Read ↗

Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS).Semantic proximity Read ↗

Modular protein expression by RNA trans-splicing enables flexible expression of antibody formats in mammalian cells from a dual-host phage display vector.Semantic proximity Read ↗

An improved helper phage system for efficient isolation of specific antibody molecules in phage displaySemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.