Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Expression and detection of ScFvB9 and its mutant in recombinant phage antibody system.

Hybridoma and hybridomics - 1 Jan 2001

Yang E J, Kwak J W, Chang H C

Abstract excerpt

Recombinant single-chain antibody (ScFvB9) and its mutant (ScFvB9-6) were generated by using a polymerase chain reaction (PCR) from the Fab fragment of the murine monoclonal antibody (MAb) B9, MabB9 (gamma2b,kappa), which is specific for human plasma apolipoprotein (apo) B-100 of low density lipopreotein (LDL). In the recombinant phage antibody system (RPAS), the constructed ScFvB9 and ScFvB9-6 antibody genes...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Apolipoprotein B-100
Share to Apolipoproteins B
Share to Cloning, Molecular

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Escherichia coli

Share to Genes, Immunoglobulin

Share to Immunoglobulin Fab Fragments

Share to Immunoglobulin Fragments

Share to Recombinant Proteins

Source

Identifiers

PMID: 11839255
DOI: 10.1089/15368590152740770

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Expression and detection of ScFvB9 and its mutant in recombinant phage antibody system.PMID 11839255Read ↗

Fab is the most efficient format to express functional antibodies by yeast surface displaySemantic proximity Read ↗

Optimization and modification of anti-rhTNF-α single chain variable fragment antibody: effective in vitro affinity maturation and functional expression of chimeric Fab.Semantic proximity Read ↗

Single-chain fragment variable produced by phage display technology: Construction, selection, mutation, expression, and recent applications in food safety.Semantic proximity Read ↗

Cloning and characterization of a single-chain fragment of monoclonal antibody to ACE suitable for lung endothelial targeting.Semantic proximity Read ↗

Efficient amplification of light and heavy chain variable regions and construction of a non-immune phage scFv library.Semantic proximity Read ↗

[Construction and expression of scFv phage antibody from natural anti-keratin monoclonal antibody 3B4].Semantic proximity Read ↗

Isolation and characterization of a novel human scFv inhibiting EGFR vIII expressing cancers.Semantic proximity Read ↗

Isolation and characterization of a novel GRP78-specific single-chain variable fragment (scFv) using ribosome display methodSemantic proximity Read ↗

Affinity improvement of the fully human anti‑TSLP recombinant antibody.Semantic proximity Read ↗

Chicken monoclonal antibody isolated by a phage display systemSemantic proximity Read ↗

An efficient method for variable region assembly in the construction of scFv phage display libraries using independent strand amplificationSemantic proximity Read ↗

Affinity improvement by fine tuning of single-chain variable fragment against aflatoxin B1.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.