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Article

Quantification of the effects of mutations on receptor tyrosine kinase (RTK) activation in mammalian cells.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

He Lijuan, Hristova Kalina

Abstract excerpt

Single amino acid mutations in receptor tyrosine kinases (RTKs) are known to cause receptor over-activation and disease. Here we present a detailed protocol for the quantification of the effect of mutations on RTK activation in mammalian cells. The activation measurements are based on Western blotting, and involve direct comparison of receptor phosphorylation under conditions that ensure identical expression of...

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PMID: 25319891
DOI: 10.1007/978-1-4939-1789-1_8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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