Article
Site-directed spin labeling of a genetically encoded unnatural amino acid.
Proceedings of the National Academy of Sciences of the United States of America - 22 Dec 2009
Fleissner Mark R, Brustad Eric M, Kálai Tamás, Altenbach Christian, Cascio Duilio, Peters Francis B, Hideg Kálmán, Peuker Sebastian, Schultz Peter G, Hubbell Wayne L
Abstract excerpt
The traditional site-directed spin labeling (SDSL) method, which utilizes cysteine residues and sulfhydryl-reactive nitroxide reagents, can be challenging for proteins that contain functionally important native cysteine residues or disulfide bonds. To make SDSL amenable to any protein, we introduce an orthogonal labeling strategy, i.e., one that does not rely on any of the functional groups found in the common 20...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.